![]() A cis-acting function for the Coronavirus leader in defective interfering RNA replication. cis Requirement for N-specific protein sequence in bovine Coronavirus defective interfering RNA replication. This process is experimental and the keywords may be updated as the learning algorithm improves.Ĭhang, R. These keywords were added by machine and not by the authors. The data support the importance of the poly(A) tail in Coronavirus replication and further delineate the minimal requirements for viral genome propagation. Poly(A) tail mutants that were compromised for replication, or that were unable to replicate at all exhibited less in vitro PABP interaction. Binding of the host factor poly(A)- binding protein (PABP) appeared to correlate with the ability of DI RNAs to be replicated. Poly(A) tail extension/repair was concurrent with robust replication of the tail mutants. A BCV DI RNA lacking a poly(A) tail was unable to replicate however, a MHV DI lacking a tail did replicate following multiple virus passages. Bovine Coronavirus (BCV) and mouse hepatitis Coronavirus A59 (MHV-A59) DI RNAs with tails of 5 or 10 A residues were replicated, albeit at delayed kinetics as compared to DI RNAs with wild type tail lengths (>50 A residues). To further examine the requirement for the Coronavirus poly(A) tail, we created truncated poly(A) mutant defective interfering (DI) RNAs and observed the effects on replication. The 3′ poly (A) tail plays an important, but as yet undefined role in Coronavirus genome replication. ![]()
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |